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Synthesis of Fluorescent Silica Hollow Nanoparticles Using Coumarin Fluorphore
- There have been a lot of studies on fluorescent silica nanoparticles as a bioimaging reagent. Almost all of them, however, are based on dense silica nonoparticles. Recently, increasing number of studies have been directed to fluorescent silica hollow nanoparticles, because hollow nanoparticles can be employed as theranostic (therapeutic + diagnostic) particles. In this study, the fluorescent silica hollow nanoparticles (FSHN) have been synthesized by labelling coumarin dye on the surface of silica hollow nanoparticles (SHN). SHN has been prepared by utilizing the triblock copolymeric micelles of poly(styrene-b-2-vinylpyridine-b-ethylene oxide) (PS–PVP–PEO) as a template and tetramethyoxysilane (TMOS) has been added as a precursor. The micelles exhibit a core–shell–corona (CSC) structure in aqueous solutions. The prepared SHN has been thoroughly characterized by transmission electron microscopy (TEM) and X- ray diffraction spectroscopy (XRD). According to TEM results, the size of the silica hollow nanoparticles is about 33 nm, the size of the cavity part is about 10 nm, and the size of the shell is about 12.5 nm. The SHN is amorphous in nature. FSHN has been synthesized by labelling silane coupling reagent, 4-methyl-7-trimethylsiloxycoumarin (MTSC) on SHN. The synthesized FSHN has been examined by transmission electron microscopy (TEM). The size of the synthesized hollow silica nanoparticles is 35±1 nm and the size of the cavity part is 10±1 nm, and the size of the shell is 12.5±1 nm. Fluorescence properties of FSHN are elucidated by observing various fluorescence parameters such as intensity, fluorescence lifetime and quantum yield. The fluorescence life time is 2.08 ns and the quantum yield is 0.637. The results demonstrate that the FSHN can be successfully prepared.
Min Min Yee
- Biological and Chemical Research