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Screening of Calorie Restriction-induced Genes in the Rotifer Brachionus Plicatilis
- Rotifers play an important role in an aquatic ecosystem and are used asmodel organisms for various study areas. Although many studies have been conducted to identify environmental factors that influence rotifer populations, the molecular mechanisms involved still remain to be elucidated. The availability of food resource is also a one of the most important environmental factors that fluctuate rotifer populations over time. In this study, gene(s) differentially expressed by calorie restriction in the monogonont rotifers, Brachionusplicatilis, was analyzed, where a calorie-restricted group was fed 3 h/day and a well-fed group fed ad libitum. A subtracted cDNA library from the calorie-restricted rotifers was constructed using suppression subtractive hybridization (SSH). One hundred sixty three expressed sequence tags (ESTs) were identified, which included 109 putative genes with a high identity to known genes in the publicly available database as well as 54 unknown ESTs. A total of 38 different genes were obtained among 109 ESTs. Gene ontology study showed the differentially expressed genes related to cellular structure, transport, and division; DNA synthesis; metabolism; transcription; RNA biosynthesis; and other functions were 24, 11, 36, 5, 3, 18% respectively, whereas genes with functionally unclassified were 3%.
Aung Kyaw Swar Oo
- Journal of the Myanmar Academy of Arts and Science.